hBG5531—8本标准5用适商品于食中还粮糖和非还原糖含原量测定的。中铁氰化其钾主法要适用于小粉麦1。还原糖测定11.铁氰钾化法.1.1仪1器用具1.1.和.11磨口带锥塞瓶：形001lm；11...12量：50筒ml、52ml；.1.1.1移3液：5m管；l.111.4玻.璃漏斗；1.1..15管：试直1.8径2.0～cm，高1约cm8；.11.16.锅：铝作水浴沸用；1.1.1.7电炉：2000W；.111.8.丝笼铁放置：管用；试.1.1.19试管架1；1..11.0锥形瓶1：00ml；.111..11量滴定微管：m5或l01ml刻（度0.20m）；l1..1.121析天分平：感量00001.g。1.12试.1.1.剂2.195％乙；醇1..1.22乙酸冲液缓：m3冰l酸、6乙.8乙酸g钠比和重18.4的酸硫4.5ml，合混用水稀后至释1000ml；1..1.2132％钨钠溶液酸1：2钨g钠N酸aW24·O22H溶O于100l水m中；11.2..041N.铁化氰钾溶液：32.9g干纯净燥的铁化氰钾44与.0碳g钠溶酸于1000lm水中； h1..2.15乙酸盐液：溶70g纯化钾和氯40g硫锌溶酸水中于再加入，002m冰l酸乙再，用水释至1稀000lm混，匀；11...261％碘化0钾溶液10：纯碘化g钾于溶010ml水，再中一加滴和氢饱氧化溶液钠；.1.217.1％粉溶淀：液1可g性溶粉淀，少量用水润湿和调后，入沸倒水10m0l，继煮沸，搅拌直续至液溶清晰；11.2..801.N硫代硫钠溶液酸按（G54B098—5《粮、油料和植物油食脂验检般一规》附则录B行进配制标定和）。.1.3操作1方法1..3.1提1样品液：精取确取粉称5样.657g于10m0l口锥磨形瓶中，用5lm乙浸湿全部试醇样再，加50入m乙酸缓冲液，摇l后匀再入加2ml钨酸溶钠，在液振器荡混上合振摇5im，取出n滤过弃，最初去几滤液，滴收集滤于干液锥净瓶中形，此液滤为即样品测定液。另一取形锥不瓶试加样，上操同，滤液作为即白液。空.11.32氧.：精确吸取样化液品5ml于管中试，精再确加入5ml铁化氰溶钾，混液合立即后将管试置铁（丝笼，每中次可时作同干样若测定品）放剧入烈腾的水浴中沸并，使管液面在沸中水面下液～43cm加，热准02inm后取出立，即用冷水迅冷却。速.11..33定滴将试：管容物倾内10入0ml锥瓶形中用2，5m乙酸盐溶l洗液荡试管一并倾入锥形瓶，加5ml10％化钾，混匀碘后立即，用01.硫N代酸钠硫滴至淡定色黄，加再1ml粉溶液淀继续滴，定直溶至液蓝消失色，记用下去硫代硫酸钠溶液积（体V1。1）..31.空白4试验：吸空取白液5ml，替样品液同代.11..23和1..3.13操，作下记耗消的代硫酸硫溶钠液体积（V）。01.14.果结计算还原含量先按公糖（式）计1出算氧样化品中“还液原”糖所需.10铁N化氰钾液体积溶V（）3，根再据此积体（lm）查表1
，即可得查试样含中还原糖以（芽麦糖算）的计百分数按公。（1）式算：…计…………………………………………………1（）式中V：─0─滴空白液定消耗氰化铁溶液体钾积，m；lV1──滴样定品消耗铁氰化液溶钾液积体m，l；hV3 ──氧化样品中还原液所需糖.10N铁氰钾溶液体化积ml；，N──代硫硫酸钠液实溶当际量浓。度试双验结果许差不允过超.05％0求其平均数，即为，检结果验。验结果取检数小后点第位二。.115..10N铁化钾氰（ml）与还原含糖对量可照表查1注。还：原含量糖以芽麦糖计。算12费.氏液法1林..12仪和用器具.21.1.1古氏埚：坩52ml；1..212.滤抽：5瓶00m；l12.1.3.空泵真或泵等。水12.2.试1.2.2.1剂费林氏溶液a；碱性．酒酸铜甲液：石取酸硫铜结晶34.693g，适量水加解，加溶硫0.酸5lm，加再水至005ml用精，石棉制滤过b；．碱酒性石铜酸乙液取：石酸酒钾1钠7g3与氢氧钠5化0，加适g量溶解水稀，释至500l，用m精制棉石滤过贮存于，有具橡皮塞玻璃的内。瓶.21..2精制石棉2：用3先N酸盐将棉石泡浸～32日后，用水洗。净再加1％氢0化钠氧溶液浸2泡3日～倾去溶液，用，碱热酒石酸性铜乙浸液数小泡，时用水洗净再。以N3盐酸浸数小时，泡用水至洗不呈酸性，使成之为微的细软纤维，用水浸贮存泡于璃瓶玻内作填，充古氏埚坩用1.2。2.3.0.1N锰酸高标钾准液溶。12.2.41N.氧氢钠溶液：化取氧化氢4钠g，加溶解水至10ml。0.2.21.5硫酸溶铁：取液酸硫50g，铁加2水00ml溶解后慢，慢入硫酸加10ml，0后冷水至加000ml。1.21.2.36N盐：取盐酸酸25m，l加至水100l。1.2.3操作方法 m01N.K3/S<e(CN)Fm6lh表1原还糖％ h010.0.0203.00.400.500.60.00708.0090.1.00.10112.01.03140..5101.06.710.180.102.0029.10220.2.302.402.0256.02.07.202890.3.003.130.20.3330.403.035.0367.0.83039.040.04.04.204.130.050.100.1050.2002.0.31503.0.4610.64051.0.65.0060.650.710.670.00.858.0900961.0.1.160.1111.16.111.2261.301.351.401.54.511156..111.66617.11.71.8261.881.952.02.1072.31218..252h 440.4504..60470.4.048.059005..01.525030.5.4055.056.5.7005.05.8096.006.10620..3066.406506.60.670..806.6097.007.01.2077.30.4707.50.7067.077.08790.80.0810..280.808.340.508860.2.13.3227.42.514.5722.462.70.267.8222.882.93.052.3083.153.23.228.3433.134.4735.3.6303.73.7363.73.895.9233.98.406412..148.4254.31.34484.5451.458.4.64.724.7584.854.924.95.905 h8708..0851.52.911..231.样试理：准处称确试取10～样20g放入20m5l容量瓶，中加2水00l，在m45℃水中加热浴1，h并时时振摇，时到
待冷后水至刻度，加置静后，吸澄取清液200lm于另一502ml容量瓶中，加碱性酒酸铜甲液10石m和l1N氢氧化钠液溶4ml摇匀，加，水至刻度静，置30imn，干燥滤用过滤，弃去初纸滤液，余其滤供测液还原糖定非和还糖用。原1.23.2.滴定精：密取制备的试吸样液5溶ml于0400lm烧杯中加入，性碱酒石酸甲、乙液铜25各ml盖一表，皿面置，炉电上加热，在m4in内沸腾再，沸2mi煮，趁n用铺热有石棉的氏古坩埚或垂（坩埚）融抽滤并，用6℃0热水洗涤杯烧和淀，至沉洗不液碱性为止呈将古氏。埚放坩原回400lm烧中，杯硫酸加铁溶液52l和m2水5m，用l玻棒搅，使氧化拌铜亚完全溶解以，.10N高酸锰钾标准溶液滴定至微红。同时取水50色ml，碱性加石酸铜酒甲乙、各液2m5，l试做空剂白验。试1..42果计算结当于试样相还中糖重量原氧的化亚铜量重按式（2）公计算：＝（V－xV）0×7N.541……………………………………………………（）2中式：x──相当于试中还原糖样量重的氧化铜亚重量的mg；，V──试样消高耗锰钾标酸准溶的液体，m积l；V──0样试空消耗高白酸锰钾标溶液准的体，积l；mN──高锰酸钾标溶液的当准量度浓；1.745──1N高锰酸钾标溶液1准ml相当氧于亚铜的化克数毫。所得的由氧化铜亚重量按附录，的A表出查相当的还糖原（葡萄糖计以的）量。重还原糖干基含量公式按（）3计：算…………………………………………………（3）… h式中：A──由录附A表中查得的还原糖以葡萄（计）糖的量重，m；gW─试样─量，重；gM──样水试百分分，率；％双试结验允许差果小数点及数同1.位.41。注：沸时煮间必须控制在4min内。可先取水05l加碱m酒石性酸铜、甲乙①液各2m5l，节调适好的当火后，力再作样品。②煮沸的后液如溶不呈色蓝，表示量过糖高，减可试少样重，另作量。2还原非测定糖21.铁氰化法钾需仪器和所用同具.1.1。1需所试剂同.112。..211.操方法作分吸取别.11.3.样1品及空白液液5各ml于试中，管先在剧烈沸腾水的浴中加热51im（n样液品非中原糖转还化还为原糖），取迅速出却冷后，加入碱性铁化钾溶液氰5lm，混后匀再，入沸腾水浴中继放续热加2mi0，取出n速冷却后迅，立即1.1按3.3.法方行滴进，分别记定下滴定样液及空品白消液硫代耗硫钠体积（酸）。21..2结果算计还非原含糖量按先式（4公计），算求氧出化品样中总“还原液”所糖0.需N1氰铁钾溶液化需的体所积V4），然（后减去单氧化样独品中液“原还”糖所的需铁化氰钾溶体液（V3）积〔
公式1（〕，最后）再据根4V－3V的结果m（）l查表，2即可查得样试含中非原还（以蔗糖糖计）的百分。数……………………………………………………4）（中式：定滴白液空耗消硫代酸硫钠溶液体积ml，；────滴定品液消耗样硫代硫钠酸液体溶积，ml； hV4──化氧品样中总液还原糖需所01.N铁化氰钾溶体积，液lm； hN──硫硫代钠溶酸液当浓度量双试。结验允许果不差过超0.50，求其％平均数，为即测定果。结测结果取小数点后第二位。定21..03.1N氰化钾（铁l）与m非原糖还量对照可查表含。2：注非原还含量以糖糖计算蔗表2。01N.K3Fe(NC6</SU)ml非原还糖％ h0.010200.3.0040.05.00.00.670.8000.9010.0.111.0021.30.4011.501.601.70180.190.2.002.10.2202.302.402.502.60.702280..9023003..130.02.303340.3.503.60370.38.0.93040.0.4041.20.3040.05.1000.15.091024.0290..4303.0843.0.80.425.570.060.6270.710.76.8010.86.9100.591001..041.901.1411.9.21132.8133..1813.4134.18.521.75.6111.66.7111.71.6811.58190.1952..00.240 h.404.544.604.700.840490.5005.1.0.2505.30.5405.505.60.570.8055.0960.06.160.26.0306406.5.0.606.706.6086.97.0070.07.2170.3074.0.705.60777.07.80.9708.008.01.280.380.408.850.0292.142.1282.32.282.32.33824.224.72512.5.6261.2662..72.752.0820.082.902.492.93.9043.90.133.3183.3232.83.333.3734.23.437.253.5736.2367.3.7327.7.3283873.92.3.97.0244.07h2 .2林费氏液2法2.1试.剂2.21..16N酸：取盐盐100m酸l加，至水200lm；2..122.基红指示甲液：01.甲基％乙醇溶液红；.2.2132.0％氢氧化溶液；钠22.1..4其余试同1.剂2.。22..22操方作吸取1.法.23.1项已下制备样的品液50lm，注入1000ml容量中瓶，6N盐酸加5m，l在68～07水℃浴加热中15min，冷后却加基甲红指液示2滴，用2％0氢化氧钠液溶中，和加至水刻度混匀，，然按后12.3..2测定样品中液总原糖还。2.2.3果计结算非还糖原基干量（含以蔗糖）计按式（5公）计算：………………………………5）（式中：A──′转化后测得还原糖的（葡萄糖以）计量，重g；Vm─转化─后稀释至100l，m于用测还定原糖体积，ml；的W──测原还定原糖试样时重量，；g h.05─9还─糖（以原萄葡计糖换）算为糖蔗的数因M──；样试水分百率，％。分双验结果允许差及小数点试数同位2..2。1附A录相于当氧亚铜重化量葡萄糖的、糖、果转糖化重量表（充件补）相同于氧亚铜重化量的萄葡糖果糖、、转糖化重量下见：表化亚铜氧葡糖萄果糖mg转化糖 1.312.413.15146.1.51869.1801..910.322142.2523..64.285.292.702.8129330
..4315.3.2633.834.39.630.23873.39.4405.41.742.83.945.4460.4273.8.4494.55.0751.58295..405.52h46.5.1.5660.6.57.075..808.589..49.99104.0.19114.11.12.93128.13.313.84.311.841.351.756121..7167.2717.1.8281.71.29917.0.212.602.1211.6221.226.23.1236.5.1.566.61.772.7.8.3788.9.39.9104.0.91115.21.012.5131.316.14.124.751.21581.6.3168.1.471.71894.19.01.5920.12.0216.11.72222.22.28.3332.84.242.4925.24.605.257..26677..72.7.28879..2.9710.20.171.211.17213.1.2831.33.8114314.815..35.81163.61.817.31.871.381.8919.4199.2.042.0912.42.192.422.293.52240.2.54520 56..35.475855.9.706.8619.630.64.26.3566.47.668.76698.0.9772.137.74.237.456.6777.78.787.9981.128.23.38844.8568..768.788.980.199.219239.34.496.9.596.8797.9991.h4212..426.125.656.212.527.067.225.028.852.0992.50.030.353103.1.5320.3.5233.0335.4.30345.5.303553.60.3.537.0637.35.8038.539.03.5940.040.51.041454..2042.5430.2.62751.27.628.28.279.229.820.3330.931.431932.53..3303634..13.4735.23.58363.3.86374.7.393.5893.39.604.14007.4.121.744.32428.34.43.4944.545.450.66.4146.47.227.556.206.527.202762..812862..19296.0.13306.132.13.73.2232.73.2333.7343.438.5.333.85363.3.6873.473.938.4389.394.04.040.54.0114.5420.246.431.43.644.144.745.21 0.20101.312.05103.6041.170.8510701.8.10109.2101.31115.121.163.7114.8111.06117.11182.1913.1205.211.6122.723.18125.1026.1127.212.3128.9513061.137.123.814.3013.153612.17.4318351.3.69140.74119.1430h.4.3544.44.505.044.5546.046.5470.74.5840.8.459.049.5405050.65.11.51.62.155.6253.15.365.454.165515.5.66.155.767.255.757.25887.59259.7.60.602.6713.16.86.236.2874.84.8384.994.40.505.5051.1516.52.252.75.3533.84.55449.5.55650.566.57.15.75782.58.85.35999.604.1.06616.62.162.736.263.8463.6.49654.6.0666567.1.7.768.626.88547.4.246.677.4374.84.83488.49.49.945.045095.15.52.52.50530.5.364515..645.25557.56.25.67753.5.8785.35.9859.49.9560.416.061.56.0262.63.1636.64.2664.75.6625.8 14.411452146..414.7514.8641.7190.91552.053.1115.214554.156.5175.615.817599.161016.2.116.21634.1654.5616.667.1168.89107.017.1117.32173.41475.175.617.86177.91790.180.181.1183.24138.51485.18581.8.9h0633.6.836.3644.659.4569.6.6466.96.74680.6.58960.965.70.070.71.517167..1272.63.717.3747.274.75.275.7776.73.68773.7.877.3878.7994.9.978.48110.18.528.028583..1963.9.97604.7.107.6172.1277.372.3.784.737497.5.756.06.6777.177.778.378.87.98040.80.58.181.168.2282883..338.948.458.0856.6.81867.783.788.88.49.8098.90.51906.663.668.6746..9768.46.90965.7007.0.671.171.672272..7
73.73.827.437.497.545.7796577.0.77.6871.78.69.779.28073.808.1.381898.2.4830.83.845084..65818..576.828.86188 018..1919.0311.499125.193.6149.8195.919.0718.9199.31200.421.502027.20.820349.206.0072.228.03290.2140.512.7121.821329.215021.6.2172.238.412195.22.0722.1822.92242.220.52226.3227.224.58292.732.8h08.63481.486.582.8.5786.286.77.387.88883.8.988.4899.90.9491.091592.0.92.693.13.996.42497.9.259.759.369.68973.9799.84.9.98995.100.010.5100.11011.610.22021.71032.01.3819.29.81923.9299..539.0944.65.2995.967.396.997.489098.69..299.9710.301009.11041.200.012.1630.113.70104.3104.81054.16.0010.6610.7110711.0.8318.08109.410.10110.6111.111.71121.3121.9873.8.8887.488.99.859.000.6991.1917.92.29.829.3933.894.449.99.55960.966.9.71977.89.298.8993.99.900.141010.01.5112.1012.6003.211037.10.310448105.410..06106.517.1007.611802 .32.9123.1324.233253.23.6427.3236.872398.20492.421.23.14244.32454.246.6247.728.4824.925911.22.25253.25344255.6.2567.57.8225.98260.2116.226.2323.64246.625.672668.26.8209.6172.207123.2275273.6.74.27h10.43104.8105.41509.106.1057.017.5018.10108.610.9120.9117.0121.0181.3111.9112.4111.9121.5114.3011.411561.11.5711.261617.117.3117.18814.1819.1915.120.02106121.11.12.1272.2122.7132.13238.12.44241.911.3114.041416.11.52115.716.3116.1917.11581011..86191.211.9180.2312.09121.512.212126.13.2212.832414.215.12055.12.1612.761273.17.9218.42291.0129.621.02103.8311313.1.9132.513.133317.134.132.4813.54108.719.030918110..4110.9111.512.11121.116.3213.1114.37114.91154.116.016.111751.17.11168.218.8111.913199.120.141.21210.621.2112.21273.31238.214.4124.9251.156.1212661.7.22127.18283.128.9129.5130. 207582.770.72.812792.82.03812.582.6228372.8.8248602.87.182.82892.3290.592.6192.27293829..0529.629172.9823.29.5903.06301.73029.0430.035130..6230743.0.5309863.1.037119.31.0334.113152.31643.7.513816.h21.5521.6120.6621.71217.7218.1228.81293.2919.30.411130.11.6332.1132.117332.133813.43.134.9135.4136.0136.5137.1173.71832.31.881393139.9.140.441.0114.1612.1144.27143.2413.1844.44149.145.51640.16.6436.1130.661732.1377.31.3838191.935.140.11047.41.3114.1182.444310.14.6314.214448.145.415.91446.514.7117.4741.83184.19495.105115..601512151.8.521.4153.0153615..24154.81554.156.0156.51571.17.7155.83130.6113.213171.3.233129.3314.143.10436135..11537.361.13638.137.4138.108361.3.9113.9710.4340.81411.41420.12.64143.141.3144.317448.1454.16.04146.647.1114.77481.3148.9194.415001.50.1615.15218.12.53 1937.2309.32.2302.133242325..432.5623.6732.87329.931.0333.1323.333344.353.536.3337.6838.39
340.34011.324.3334.44345.35.6346.843749.34.039051.31.33552.354.53534.635.8553.963580.5391.60.3336.13462.5h147.214.71487.31488.419.41500.105.5151.151.7115.2212.8155.43531.914.55515.151.5616.2556.11857.315.97518.551.90591.6160.160.7261.1163.1162.9513.06163.16462.146.715.3166.91566.516.7061.761862.186.15889.591.561.0110.6167131.1.6962.51631.163.11764.316.9416.45661.016.6167.61627.8618.416.01699.17602.7108.17.14712.017.2617.32731.817.4471.0175.65167.21768.17.741870.7186.17.29197.8180.411.0818.1612.951535.1541.51.6451.5215.5815.4156.0757.511851.51.871593.15.99160.51160.161.6126216.2.8631.46140.164.565.11165.716.63166.917.561680.168.619.2616.91780.141.0177.16721.217.28713.373.9117.5475.1136 .36643.865.3936.7308.263963.307.471353.727.73.83347.9376.077.323783.379.480.3538.713828.383.9385.03862.83.3738843.89.59037.3198.39.9293.4039.523963.37.493895.99374.0.0804.1943.010442.405.304.46h16.9316.9917.501711.1716.72.1172.281734.731917.4.5751.17157.1673.17.6817.4718.70187.61792.17.91807.3180.281151.82.11287.1382.18.3818.4415.081856.1862.81.68871.318.9718.51889.119.7890.1391.9109.51812.2182.813.84148.014.681852.18.58186.14870.187.6188.218.8881.9149.1090.71191.311.9912.95193.11397194.3.149.9915.519.6116.71997.317.9998.5119.29199.820.024010.20.61202.2202.28034.04.20204.702.5315.77761.316.79177.517.181877.791.217.819804.811.180.1618.221828.18.3414.8014.6818.2518.5188.4617.0887.611882.1888.19.8410.9019.016912191..8912.4193.19306.19421.4.8195.4996.0196.6119.279178.18.49 47.0604874.9.0814.09412.1431.241.34145.41466417..71488.149.921.1442.242233.2444.45.264267.472.8428.4903.143.2413.32433.54346.435.47638.483.034.9441.24410.3442.5443.644.47445.84470.4481.44.9240.53h192.0921.691.231938.914.1495.019.6195.261968.19.47198.0198.19951.991.207.0320.90201.520.2102272.033203..92405.205.21057.26.3020.69207520.81.20.7820.3920.992105.1211.21.127123.12.29213.5241.21147.20.59062.250.7217.7020.820390.209.62012.12.082114212..202.6121.332319.12.542151.21.52176.3271.012.7261.28218.829.5122.01220.722.1223.1922.262322.22.3822.4422.15225.722.322669.227.6228.222.882294.19.09199.620.20020.280.412020.02262.3.02203.2840.4205.025.7020.63026.2079.520.812087209.3.09.29102.512.11211.821.422310.132.621422.1.48125.4216.016.7221.37217.912.521981.2918.2204.221022..621222. h415.542.65534745.4.45680.47.51548.259.3464.5046.616427.6438.64.0566.4147.6462.4869454.0.76741.74279.4470.475.174.6277444.7.547896480..741.8943.0488.4485.1482.64487.458.648897.215.31259.26.5217.112178.28.411290.219.62202.202.822
1.422.0222.622233.223.924.5225.122527.22.36272.02726.22.822288.292.250.13320.731.242230.232.73233.34.0224.73352.33621.32.9632.1023.702313.32.2022.3623.2233.39234.53521.235.8236.234712.3.7237.4829302.397.2403.241024.1.622.2424.2943.26244.324.4245.926463.247.2074.8482.529.2452.0050.822156.522725.3.272.9222352.4.1224272.5242.26.206.262722.27.9222.5829.122297.23.423100.32.12327.3223.293.6234.233428.25.533621.263.823.5738.1232.883925.2402240.8.2415.42.224330.2438244..724.85：摘注自食品卫《生验方法检理化部分2》63242页。～h 加说附明：本准标由中华民共和人商国业提出部。本标准由业商粮部储运局负食起责草。标准本主要草人高起修、杨浩吾然、吴霞、艳吕桂。芬迎您欢的下，资载仅料供考！参
发布于 2025-01-15 15:01:25
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